Regulatory

Part:BBa_K4115027:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)


PcstA_Mutant2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A mutant of PcstA.

Source

The original version of PcstA was amplified from E. coli DH5-alpha genome by PCR. The mutations are added through PCR.

References

[1] Soberon-Chavez, G., Alcaraz, L.D., Morales, E., Ponce-Soto, G.Y., and Servin-Gonzalez, L. (2017). The Transcriptional Regulators of the CRP Family Regulate Different Essential Bacterial Functions and Can Be Inherited Vertically and Horizontally. Front Microbiol 8. ARTN 959 10.3389/fmicb.2017.00959.
[2] Shimada, T., Fujita, N., Yamamoto, K., and Ishihama, A. (2011). Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources. PLoS One 6, e20081. 10.1371/journal.pone.0020081. [3] Gasperotti, A., Going, S., Fajardo-Ruiz, E., Forne, I., and Jung, K. (2020). Function and Regulation of the Pyruvate Transporter CstA in Escherichia coli. Int J Mol Sci 21. 10.3390/ijms21239068.